Prevalence and Molecular Characterization of Porcine Parvovirus 2 in Southwest China During 2020–2023

Figures

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  Figure 1. The epidemiological study of porcine parvovirus 2 in the southwestern region of China. (a) A serological investigation of PPV2; (b) a phylogenetic analysis of the full genome of PPV2.

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  Figure 2. IFA of the PPV2 SC2020 strain. 4′,6-diamidino-2-phenylindole (DAPI): DAPI was utilized for staining cell nuclei. Fluorescein isothiocyanate (FITC): following the binding of the anti-VP2 protein mouse polyclonal antibody to the PPV2 VP2 protein, FITC-labeled goat anti-mouse immunoglobulin G (IgG) was utilized to bind to the complex, resulting in the emission of green fluorescence. Merge: combine DAPI and FITC staining pictures.

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  Figure 3. Phylogenetic tree analysis of the PPV2 SC2020 strain. The reliability of the tree was evaluated using the maximum likelihood method, employing the Tamura Nei model and performing 1000 bootstrap repeats. (a) Phylogenetic analysis was performed based on the PPV2 ORF1 gene; (b) phylogenetic analysis was conducted based on the PPV2 ORF2 gene.

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  Figure 4. Recombination analysis of the PPV2 SC2020 genome. (a) The genetic similarity between the recombinant strain and its parental strain was assessed using SimPlot. The PPV2 SC2020 genome served as the query sequence. Recombination breakpoints were denoted by red lines, with their position indicated at the bottom. (b) The phylogenetic analysis was conducted based on nucleotide (NT) regions 1–1117 nt, 1548–3017 nt, and 4694–5179 nt. (c) The phylogenetic analysis was conducted based on nucleotide (NT) regions 1118–1547 nt and 3018–4693 nt.