Key result
New C4B6 antibody ELISA specifically detects human Des-gamma-Carboxy-Prothrombin forms with ~0.16 A.U/ml minimum sensitivity.
Why the study?
Monoclonal antibodies specific to human Des-gamma-Carboxy-Prothrombin (DCP) were produced to explore pathologies possibly associated with vitamin K deficiency.
Population
In vitro production of monoclonal antibodies against human DCP
Comparison
Monoclonal antibody C4B6 vs other antibodies for DCP detection
Design
Preclinical development and standardization of ELISA test
Authors
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May support DCP assay research; leaves open clinical validation and diagnostic utility in patients.
The development of a sensitive ELISA test using mAb C4B6 allows for the specific detection of human Des-Gamma-Carboxyprothrombin in the presence of calcium ions.
Belle et al. (1995) studied Vitamin K deficiency. Enzyme-Linked-Imunosorbent Assay (ELISA) using mAb C4B6 was evaluated on Sensitivity and specificity for DCP forms. An ELISA using the new monoclonal antibody C4B6 was developed and proven specific for human Des-gamma-Carboxy-Prothrombin forms with a minimum sensitivity of 0.156 A.U/ml.
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