A new method for the preparation of an immunologically homogeneous β-casein

Summary The preparation of an immunologically homogeneous β-casein is described, involving several separations by chromatography on diethylaminoethyl cellulose with urea-imidazole buffer at pH 7, followed by Tris buffer at pH 8·2. 30% of the β-casein present in the skim-milk is obtained by this method. β-casein gives a single band in urea-starch gel electrophoresis when it is obtained from the milk of cows homozygous for this character.