Programmed Cell Death-1 Expression in T-Cell Subsets in Chickens Infected with Marek’s Disease Virus

Figures

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  Figure 1. CD4+ T-cells transformed by Marek’s disease virus express a high proportion of programmed cell death 1. The expression of PD-1 and Meq in CD4+ T-cells was analyzed (control group: n = 10, spleen of MDV-infected chicken: n = 13, tumor tissue of MDV-infected chicken: n = 13). (A) The proportions of Meq+ cells in CD4+ T-cells in the spleen and tumor tissues were analyzed. (B) Representative flow cytometry plots showing PD-1 and Meq expressions in CD4+ T-cells. (C) The proportions of PD-1+ cells in the total CD4+ cells in the spleen and tumor tissues were compared. (D) The proportions of PD-1+ cells between Meq+ CD4+ and Meq− CD4+ cells in the spleens of uninfected and infected chickens were compared. The red line indicates the mean value. Asterisks indicate significant differences (** p < 0.01, * p < 0.05; Dunn’s test between the groups; Wilcoxon signed-rank sum within the same individual).

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  Figure 2. Expressions of interferon-γ and programmed cell death 1 in CD8+ T-cells in Marek’s disease-developed chickens. The expressions of PD-1 and IFN-γ in CD8+ T-cells were analyzed (control group: n = 10, spleen of MDV-infected chicken: n = 9, tumor tissue of MDV-infected chicken: n = 9). (A) The proportions of IFN-γ+ cells in the total CD8+ T-cells from the spleen and tumor tissues were determined after unstimulated and stimulated culture. (B) Representative flow cytometry plots showing the upregulation of PD-1 and IFN-γ in CD8+ T-cells. (C) The proportion of PD-1+ cells in the CD8+ T-cell population from the spleen and tumor tissues was analyzed. (D) The proportions of IFN-γ+ cells between PD-1+ CD8+ and PD-1− CD8+ T-cells in the spleens of uninfected and infected chickens were compared. The red line indicates the mean value. Asterisks indicate significant differences (** p < 0.01, * p < 0.05; Dunn’s test between the groups; Wilcoxon signed-rank sum within the same individual).

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  Figure 3. Expressions of interferon-γ and programmed cell death 1 in γδ T-cells in Marek’s disease-developed chickens. The expressions of PD-1 and IFN-γ in γδ T-cells were analyzed (control group: n = 10, spleen of MD virus (MDV)-infected chicken: n = 9, tumor tissue of MDV-infected chicken: n = 9). (A) The proportions of γδ T-cells expressing IFN-γ in the spleen and tumor tissues were determined after unstimulated and stimulated culture. (B) Representative flow cytometry plots showing the upregulation of PD-1 and IFN-γ in γδ T-cells. (C) The proportion of PD-1+ γδ T-cells in the spleen and tumor tissues was determined. (D) The proportions of IFN-γ+ cells between PD-1+ and PD-1− γδ T-cells in the spleens of uninfected and infected chickens were compared. The red line indicates the mean value. Asterisks indicate significant differences (** p < 0.01, * p < 0.05; Dunn’s test between the groups; Wilcoxon signed-rank sum within the same individual).