Key result
Overexpressing miR-223-3p inhibits myocardial inflammation and apoptosis in preclinical models by targeting FBXW7.
Why the study?
Myocardial infarction leads to myocarditis and cardiomyocyte apoptosis, and the regulatory mechanism of miR-223-3p on these processes after MI was unclear.
miR-223-3p reduces myocardial inflammation and apoptosis after myocardial infarction by targeting FBXW7 in preclinical models.
Does not yet support miR-223-3p modulation in post-MI care; leaves open its role in apoptosis pathways for future study.
Background: Myocardial infarction (MI) is one of the main causes of disability and death in the world, leading to myocarditis and cardiomyocyte apoptosis. Studies have shown that microRNA (miRNA) is involved in myocarditis and apoptosis. The main purpose of this study was to explore the regulatory mechanism of miR-223-3p on myocarditis and apoptosis after MI. Methods: We cultured H9c2 cells and detected the expression of miR-223-3p in cells treated with different concentrations of H 2 O 2 . Sprague Dawley (SD) rats were fed with normal diet, constructed an MI model and detect the expression of miR-223-3p in heart tissue. Overexpression or inhibition of miR-223-3p was conducted in MI model cells in vitro , and the contents of the inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) in cell supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Cell apoptosis was detected by flow cytometry. Luciferase activity assay was used to detect the targeting relationship between miR-223-3p and FBXW7 , and the expression of FBXW7 in cells was also detected. Overexpression of miR-223-3p was performed in MI rats to detect the expression of inflammatory factors, FBXW7 , and apoptosis in rat cardiac tissue. Results: The expression of miR-223-3p was down regulated in MI models established in vitro and in vivo . Overexpression of miR-223-3p can inhibit inflammatory response and apoptosis in H9c2 cells and cardiac tissues. It was revealed that miR-223-3p can inhibit the expression of FBXW7 , reduce myocarditis and apoptosis after MI, and improve cardiac function. Conclusions: It is possible that miR-223-3p reduces myocarditis and apoptosis after MI and improves cardiac function by targeted inhibition of FBXW7 expression.
No takes yet. Share an insight, caveat, or question.
Zhang et al. (2022) studied Myocardial infarction. Overexpression of miR-223-3p vs. Control/MI model without overexpression was evaluated on Myocardial inflammation and apoptosis. Overexpression of miR-223-3p inhibited inflammatory response and apoptosis in H9c2 cells and rat cardiac tissues by targeted inhibition of FBXW7 expression.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: