Influence of Enzymatic Agents on the Ginkgo Fruit Ratio using Nano-Processing Technology

The ginkgo tree (Ginkgo biloba), which is common in Korea, is an example of a "living fossil" that existed on earth approximately 350 million years ago.It is a deciduous tree of Chinese origin.The bark is gray or grayish brown with thick cork and deep vertical cracks.Leaves appear gathered on the short branches.The leaves are often fan-shaped and divided in two.There is a fine wavy pattern on the edge of the leaf, but both sides are plain yet majestic.Ginkgo is a migratory plant being the sole species in the genus, but has eight varieties.It is difficult to identify gender in young plants, making it difficult to selectively plant trees because ginseng gender can only be distinguished after approximately 15 years.The ginkgo genders grow as separate trees.In April-May, flowers of approximately 3mm bloom widely over the tree.The number of male flowers is 1-5, the female flowers are green and there are 6-7.Two pedicels each have 2cm peduncles, but only one of them grows and ripens in October.The fruit of ginkgo, which is ripe in the fall, is oval to round with a fleshy shell for an outer skin with a pulp inside, and the flesh of the surface of the fruit has an unpleasant odor because of the ingredients bilobol and ginkgoic acid.It can cause irritation to human skin.Because of this odor and potential contact inflammation, other animals do not eat the ginkgo nut; Abstract: We investigated the effects of a preparation by wet nanotechnology on the pollen, pistil, and fruit loss in ginkgoes.Pollen germination in the test medium was investigated by adding insoluble calcium and magnesium nano particles.The germination rate increased 30% after 72 h; however, pollen in germination medium to which 1 µL agitating agent was added did not germinate after 72 h.Thus, the nanoized emulsifier inhibited germination of ginkgo pollen.Fruit loss rate for ginkgo seedlings sprayed with nanoized reding agent decreased by 60-90% more than that of unsprayed fruit.However, 4 days following the application of the re-deposition agent, the content of calcium and magnesium in the ginkgo pistils decreased, requiring reapplication within 3 days.The soil pollution test confirmed the enrichment agent did not affect surrounding soil, and it will be applicable to various roadside trees in addition to ginkgoes.Because the external environment may affect success (e.g., spraying method, rain, and wind),it is necessary to set a spraying and reapplication time.The wet nano-process technology is expected to be applicable to various fields,and can be used in natural materials and bioindustries, as well as for plant nutrients.