What question did this study set out to answer?

To identify the cellular origin of neurotoxic, proliferating astrocyte-like cells that surround motor neurons during paralysis progression in an ALS model.

January 1, 2013Frontiers in Cellular NeuroscienceOpen Access

Phenotypic transition of microglia into astrocyte-like cells associated with disease onset in a model of inherited ALS

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To identify the cellular origin of neurotoxic, proliferating astrocyte-like cells that surround motor neurons during paralysis progression in an ALS model.
Analyzed proliferation and phenotypic glial markers (Ki67, GFAP, Iba1, CD163) in spinal cords of symptomatic SOD1(G93A) rats using immunohistochemistry.
Cultured spinal cord cells from symptomatic rats, sorted CD11b-positive microglia by flow cytometry, and monitored marker expression and forskolin-stimulated differentiation over two weeks.
Ki67-positive aberrant astrocyte-like cells expressing both astrocytic (GFAP) and microglial markers (Iba1, CD163) increased sharply around motor neurons upon paralysis onset.
Sorted CD11b-positive microglia transitioned into aberrant astrocyte-like cells within two weeks in vitro, losing microglial markers while upregulating GFAP and S100β in a forskolin-enhanced manner.

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Implication

Experimental study reveals proliferating microglia transform into neurotoxic astrocyte-like cells in ALS rats, highlighting a novel target to slow paralysis progression.

Cite This Study

Trías et al. (2013) studied this question.

synapsesocial.com/papers/6ab0861f121bdbdd1ff81baf https://doi.org/10.3389/fncel.2013.00274

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Implication

Experimental study reveals proliferating microglia transform into neurotoxic astrocyte-like cells in ALS rats, highlighting a novel target to slow paralysis progression.

Phenotypic transition of microglia into astrocyte-like cells associated with disease onset in a model of inherited ALS

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Discussion

Implication

Cite This Study

Phenotypic transition of microglia into astrocyte-like cells associated with disease onset in a model of inherited ALS

Key Points

Discussion

Implication

Cite This Study

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