In an attempt to elucidate the relation between structure and electrophoretic behavior, the effect of pH, surfactant and buffer on electrophoretic mobilities and resolution of several flavonoids, differing in structure, has been investigated. Ring-B substitution and the presence of a free hydroxy group at C7 of ring A play an important role, whereas O-methoxylation is less significant. Glycosylation also contributes in determining different degrees of complexation with borate, thus affecting the mobilities.
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Pietta et al. (1994) studied this question.
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