Calcium Hydroxide Upregulates Interleukin-10 Expression in Time Dependent Exposure and Induces Osteogenic Differentiation of Human Umbilical Cord Mesenchymal Stem Cells

Background: Human umbilical cord mesenchymal stem cells (HUCMSCs) as one of mesenchymal stem cells source, a promising potential for regenerative dentistry. Calcium hydroxide, a widely administered medicament for therapy, is used for its numerous benefits. The presence of calcium hydroxide is expected to bring a outcome on HUCMSCs. : This study aims to evaluate the anti-inflammatory effect through Interleukin-10 expression and osteogenic of calcium hydroxide on HUCMSCs. : This study is an experimental laboratory research with controlled group design. HUCMSCs were grown Minimum Essential Medium (MEM) Alpha containing 0.1 µg/ml of calcium hydroxide. Observation of interleukin-10 were conducted in 24 hours, 72 hours, and 168 hours. Osteogenic differentiation was observed with Red S staining. Obtained data were analyzed with analysis of variance (ANOVA). The level of significance set at 0.05. : Calcium hydroxide increases Interleukin-10 expression of HUCMSCs (P 0.000). Exposure time of 24 hours, 72 hours, and 168 hours exhibit increased expression. Exposure of 168 hours displayed the highest increase, by 72 hours and 24 hours as the lowest. Calcium hydroxide also induce osteogenic differentiation of . : hydroxide increases the expression of Interleukin-10 in time dependent exposure and induces osteogenic of HUCMSCs. The longer the exposure of calcium hydroxide to HUCMSCs, the higher the of interleukin-10. These findings support the use of calcium hydroxide on HUCMSCs for pulpal and tissue regeneration.