Key result
PCR outperforms virus isolation in detecting MDV and REV in tumour-bearing and immunodeficient birds.
Why the study?
Rapid and sensitive methods to diagnose and differentiate Marek's disease virus and reticuloendotheliosis virus infections in chickens and turkeys were needed.
Population
Normal and tumour-bearing birds from 16 chicken and turkey flocks and 30 normal grandparent light breeding birds
Comparison
PCR vs virus isolation, ELISA for REV antibodies, and histopathology
Design
Observational study
Authors
Loading...
PCR enables rapid MDV-REV differentiation; leaves open routine diagnostic adoption pending validation.
Observational
PCR is an efficacious and sensitive method for the differential diagnosis of Marek's disease and reticuloendotheliosis in birds.
Davidson et al. (1995) conducted an observational in Marek's disease and reticuloendotheliosis. Polymerase chain reaction (PCR) vs. Virus isolation was evaluated on Detection of MDV and REV sequences. Compared to virus isolation, PCR was more sensitive in detecting Marek's disease virus and reticuloendotheliosis virus sequences in tumour-bearing and immunodeficient birds.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: