Key result
ADP pre-addition inhibits the initial Pi burst during Mn(II)-ATP hydrolysis only below 10 degrees.
Why the study?
The temperature-dependent behavior of the myosin-product intermediate during Mn(II)-ATP hydrolysis by heavy meromyosin from rabbit psoas muscle was not fully understood.
Population
Heavy meromyosin from rabbit psoas muscle
Comparison
Mn(II)-ATP hydrolysis below and above 10 degrees with or without ADP pre-addition vs Mg-ATP hydrolysis at 8 degrees
Design
Preclinical biochemical study
Authors
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No immediate clinical implications; leaves open temperature effects on human cardiac myosin kinetics.
The study demonstrates a temperature-dependent transition of the myosin-product intermediate during Mn(II)-ATP hydrolysis in rabbit psoas muscle.
Tawada et al. (1975) studied this question. Temperature variation and ADP pre-addition was evaluated on Initial burst of Pi liberation during Mn(II)-ATP hydrolysis. Below 10 degrees, the initial burst of Pi liberation during Mn(II)-ATP hydrolysis by heavy meromyosin was inhibited by pre-addition of ADP, whereas it was not inhibited above 10 degrees.
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