Key result
2D electrophoresis reveals an extra myosin light chain 1 protein spot in ~60% of DCM patients.
Why the study?
Differences in heart muscle protein expression in human cardiomyopathies compared to normal hearts were not fully characterized.
Does two-dimensional electrophoresis identify differences in heart muscle proteins between normal individuals and those with cardiomyopathies?
Case-Control (n=34)
Does two-dimensional electrophoresis identify differences in heart muscle proteins between normal individuals and those with cardiomyopathies?
Two-dimensional electrophoresis identified an additional protein spot in the myocardial myosin light chain 1 area in a subset of patients with dilated cardiomyopathy.
May indicate protein differences in dilated cardiomyopathy; leaves open diagnostic utility pending validation.
Human heart muscle proteins have been analyzed by two-dimensional electrophoresis. Twenty five autopsy heart muscle samples obtained from individuals who had died in accidents and who had no signs of cardiovascular pathology have been compared with biopsy and autopsy myocardium samples of patients with: dilated cardiomyopathy (5 cases), hypertrophical cardiomyopathy (2 cases) and myocarditis (2 cases). In dilated cardiomyopathy in 3 out of 5 cases an additional protein spot was found in the myocardial myosin light chain 1 area.
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Kovalyov et al. (1990) conducted a case-control in Cardiomyopathies and myocarditis (n=34). Cardiomyopathy and myocarditis vs. Normal autopsy heart muscle samples was evaluated on Protein spot differences on two-dimensional electrophoresis. In patients with dilated cardiomyopathy, two-dimensional electrophoresis revealed an additional protein spot in the myocardial myosin light chain 1 area in 3 out of 5 cases compared to normal controls.
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