Key result
Sequencing and amino acid analysis identify 12 proteins for a human myocardial 2-DE database.
Why the study?
Identification of proteins in the human myocard two-dimensional gel electrophoresis pattern was needed to establish a protein database.
This study established the basis of a human myocard 2-DE database by identifying twelve proteins using sequencing and amino acid analysis.
Hypothesis-generating for myocardial 2-DE databases; leaves open translation from animal models to human validation or clinical use.
N-Terminal sequencing, internal sequencing and amino acid analysis were used to identify twelve proteins of the human myocard two-dimensional gel electrophoresis (2-DE) pattern. Amino acid analysis was shown to be a powerful tool in addition to sequencing. The identification of a disease-associated N-terminally blocked protein by internal sequencing was not successful. The twelve identified proteins are the basis of a human myocard 2-DE database.
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Jungblut et al. (1992) studied Human myocard proteins. Two-dimensional gel electrophoresis and sequencing techniques was evaluated on Identification of human myocard proteins. N-terminal sequencing, internal sequencing, and amino acid analysis successfully identified twelve proteins to form the basis of a human myocard two-dimensional gel electrophoresis database.
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