Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

This is the authors' abstract. We don't add key points for this paper.

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.