Key result
Depletion of hnRNP R facilitates stress and TDP43 granule formation and impairs resolution in vitro.
Why the study?
The role of hnRNP R in stress granule formation and its impact on mRNA dynamics and TDP43 interactions was not fully understood.
hnRNP R plays an important role in regulating stress granule formation and resolving, as well as TDP43 granule dynamics.
hnRNP R isoforms in stress granules may modulate RNA responses to cellular stress; leaves open relevance to cardiovascular disease models.
ABSTRACT hnRNP R (Heterogeneous nuclear ribonucleoproteins R) is one member of RNA-binding proteins (RBPs) in hnRNP family, it plays important role in nucleic acid metabolism including alternative splicing, mRNA stabilization, transcriptional and translational regulation, and mRNA transport. Mostly hnRNP R is localized in nuclear and extensively binds with RNA and chromatin, but still some part of it exports to cytosol. We found that long and short isoform hnRNP R can located in stress granules, depletion hnRNP R can facilitate the stress granule formation and accelerate mRNA in the stress granules. Meanwhile we found that depletion hnRNP R can alter the stress granule phenotype and impair stress granule resolving. Additionally, we also found that depletion hnRNP R also induce TDP43 granules formation. Furthermore, depletion hnRNP R can impair TDP43 binding with ribosome. Altogether, hnRNP R plays important role in the stress granule formation.
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Changhe Ji (2022) studied Stress granule formation. hnRNP R depletion vs. Control cells was evaluated on Stress granule formation and phenotype. Depletion of hnRNP R facilitates stress granule formation, alters their phenotype, impairs their resolving, and induces TDP43 granule formation in vitro.
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