Key result
Novel formaldehyde pinpoint permeation improves sinus node dysfunction modeling success with lower mortality in rabbits.
Why the study?
A credible chronic sinus node damage model is important for studying Sick Sinus Syndrome, but optimal methods for establishing such models require evaluation.
Does the formaldehyde pinpoint pressing permeation method improve the establishment of a chronic sinus node damage model in rabbits compared to the traditional formaldehyde wet compressing method?
Does the formaldehyde pinpoint pressing permeation method improve the establishment of a chronic sinus node damage model in rabbits compared to the traditional formaldehyde wet compressing method?
The formaldehyde pinpoint pressing permeation method is a superior and more stable technique for establishing a chronic sinus node damage model in rabbits compared to traditional methods.
Improves rabbit models of sinus node dysfunction; leaves open translation to clinical research.
Sick Sinus Syndrome is a common and refractory arrhythmia, needing further study in which setting up a credible sinus node damage model is important. To explore the feasibility and superiority of an original formaldehyde pinpoint pressing permeation (FPPP) method for building a chronic sinus node damage (CSND) model, 5 rabbits were chosen from 35 as a sham-operation group, and the remaining were randomly divided into two groups: the formaldehyde wet compressing (FWC) group, in which models were established by applying a cotton bud dipped in 20% formaldehyde onto the sinus node (SN) area, and the FPPP group, in which models were established by injecting formaldehyde into the SN area through a self-made pinpointing and injecting electrode. We found that in both groups, the HR at 2 h, 24 h, 1 wk, and 2 wk after modeling decreased compared with premodeling; sinoatrial conduction time, sinus node recovery time, and corrected sinus node recovery time were prolonged compared with premodeling. The indexes mentioned shortened by 2 wk after modeling compared with 2 h in the FWC group, whereas they were stable after modeling in the FPPP group. The modeling achievement ratio in the FPPP group was higher and the death rate was lower. Under light microscope, paraffin sections of the SN tissue and cells showed severe injury in both groups. The results indicate that the CSND models in rabbits can be successfully established by the FPPP method, with higher achievement ratio, lower death rate, better stabilization effect, and less damaging comparing with the traditional method.
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Liu et al. (2012) studied Sick Sinus Syndrome (animal model) (n=35). Formaldehyde pinpoint pressing permeation (FPPP) method vs. Formaldehyde wet compressing (FWC) method was evaluated on Modeling achievement ratio, death rate, and electrophysiological stabilization. The formaldehyde pinpoint pressing permeation method successfully established chronic sinus node damage models in rabbits with a higher achievement ratio and lower death rate than the traditional method.
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