Key result
Novel fiber-based ELISA achieves ~94% agreement with commercial assays for FAdV-8 antibody detection.
Why the study?
A rapid serology test kit specific to fowl adenovirus serotype 8 was not available to detect antibodies in infected or vaccinated chickens.
A newly developed fiber-based ELISA provides a specific and sensitive tool for detecting antibodies against fowl adenovirus serotypes 7 and 8 in chickens.
An outbreak of inclusion body hepatitis caused by fowl adenovirus serotype 8 (FAdV-8) has caused significant economic losses in the poultry industry worldwide. However, a rapid serology test kit specific to FAdV-8 is not available to date. We developed a fiber-based ELISA using the purified GST-fiber of FAdV-8 as coating antigen to measure antibodies against FAdV-8. Specificity analysis showed that our ELISA could react with sera against FAdV-7, -8a, and -8b, but not with sera against the other pathogens tested. Moreover, detection of positive sera with our ELISA had 83% and 94% agreement with an indirect immunofluorescence assay (IFA) and a commercial ELISA from BioChek, respectively. Our ELISA was also effective in the detection of antibodies against FAdV-8 in sera from both experimentally infected and clinically vaccinated chickens. Our FAdV-8 fiber-based ELISA can be a valuable tool to specifically and sensitively detect antibodies against FAdV-7 and/or -8 in infected or vaccinated chickens.
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Lü et al. (2020) studied Inclusion body hepatitis caused by fowl adenovirus serotype 8 (FAdV-8). Fiber-based ELISA using purified GST-fiber of FAdV-8 vs. Indirect immunofluorescence assay (IFA) and a commercial ELISA from BioChek was evaluated on Agreement with an indirect immunofluorescence assay (IFA) and a commercial ELISA (83% and 94% agreement). A novel fiber-based ELISA using purified GST-fiber of FAdV-8 demonstrated 83% and 94% agreement with an indirect immunofluorescence assay and a commercial ELISA for detecting FAdV-8 antibodies.
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