Presents a protocol for isolating lipid rafts and characterizing their lipid composition in cultured mammalian cells, facilitating research into membrane signaling platforms.
Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.
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Mukhamedova et al. (2020) studied this question.
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