Protective effects of curcumin and beta-carotene on cisplatin-induced cardiotoxicity: An experimental rat model

Figures

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  Figure 1. Representative photomicrographs of cardiac immunostaining for IL-1β in experimental groups. (a) Control group (b) Sesame oil group demonstrated weak IL-1β immunoreaction (c) Cisplatin group showing dense IL-1β expression (d) Only β-carotene treated group (e) cisplatin+β-carotene group showing mild IL-1β expression (f) Only curcumin-treated group (g) cisplatin+curcumin group treated group showing mild staining of IL-1β in myocardial fibers. IL-1β immunohistochemical staining of heart samples from the experimental groups (Original magnification=40x, Scale bar=100 µm)

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  Figure 2. Representative photomicrographs of cardiac immunostaining for IL-6 in experimental groups. (a) Control group (b) Sesame oil group demonstrated weak IL-6 immunoreaction (c) Cisplatin group showing more denser IL-6 expression (d) Only β-carotene treated group (e) cisplatin+ β-carotene group showing mild IL-6 expression like (f) Only curcumin-treated group and (g) cisplatin+ curcumin group. IL-6 immunohistochemical staining of heart samples from the experimental groups (Original magnification=40x, Scale bar=100 µm)

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  Figure 3. Representative photomicrographs of cardiac immunostaining for TNF-α in experimental groups. (a) TNF-α immunoreactivity was weak in the control group (b) Sesame oil group. (c) TNF-α immunoreactivity intensity of myocardial fibers in cisplatin group was higher than the control group (d) TNF-α immunoreactivity of only β-carotene treated group (e) cisplatin+β-carotene group was lower when the compared to cisplatin group (f) Only curcumin-treated group (g) cisplatin+ curcumin group showed TNF-α immunoreactivity decreased in the cardiac tissues compared to cisplatin group. TNF-α immunohistochemical staining of heart samples from the experimental groups (Original magnification=40x, Scale bar=100 µm)

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  Figure 4. The morphology of the heart tissue. The images were representatives the H&E–stained sections of heart from experimental groups (a) Sagittal sections of the rat cardiac tissue showing the normal structure in control group (b) Only sesame oil treated group (cc) Cisplatin-injured cardiac tissue obtained from cisplatin group (d) Only β-carotene treated group (e) The cardiac tissue obtained from the cisplatin+ β-carotene group treated with β-carotene (f) Only curcumin-treated group (g) The cardiac tissue obtained from cisplatin+ curcumin group treated with curcumin (Original magnification=40x, Scale bar=100 µm) Thick arrow, hemorrhage Arrow, interstitial edema Star, muscle fiber damage and cytoplasmic vacuolization