Key result
Adenovirus infection disrupts surface HLA class I and CD3 but leaves La protein expression intact.
Why the study?
The mechanisms by which autoimmune responses to the La autoantigen are evoked remain largely obscure, including the role of cell surface presentation during stress conditions such as adenovirus infection.
Does adenovirus infection induce cell surface presentation of the La autoantigen or affect membrane glycoproteins in human cell lines?
Does adenovirus infection induce cell surface presentation of the La autoantigen or affect membrane glycoproteins in human cell lines?
Adenovirus infection downregulates HLA class I and CD3 antigens but does not induce cell surface presentation of the La autoantigen in human cell lines.
Answers are grounded in this paper and cite their sources.
Adenovirus does not induce detectable La surface presentation on cell lines; leaves open whether viral stress contributes to anti-La autoimmunity.
Antibodies to the RNA polymerase III transcription termination factor La are frequently found in the serum of patients with various autoimmune diseases. The mechanisms by which autoimmune responses are evoked remain largely obscure, but the presentation of autoantigens on the cell surface during stress conditions has been reported as a possible factor. In this study we analysed the effects of adenovirus infection on the binding of anti-La antibodies to the surface of several human cell lines and on the levels of the membrane-expressed glycoproteins HLA class I, CD44 and the CD3 complex. In addition, we studied the relative amount and the intracellular distribution of the La protein as well as its association with the major species of non-coding virus-associated (VAI) RNA. While immunofluorescence patterns revealed a redistribution and possibly cell surface expression of the La protein during infection, this could not be confirmed by other techniques. In contrast, surface levels of HLA class I proteins and CD3 complex were severely affected. The data suggest that the subcellular distribution of the La protein is not detectably influenced by adenovirus infection.
No takes yet. Share an insight, caveat, or question.
Peek et al. (1994) studied this question. Adenovirus infection was evaluated on Binding of anti-La antibodies to the cell surface and levels of membrane-expressed glycoproteins HLA class I, CD44 and CD3 complex. Adenovirus infection severely affected surface levels of HLA class I proteins and the CD3 complex, but did not detectably influence the subcellular distribution or cell surface expression of the La protein.
Synapse has enriched 10 related papers. Consider them for comparative context: