Key result
Novel Lp(a) ELISA demonstrates comparable accuracy to established immunoassays for standard quantitation.
Why the study?
A monoclonal antibody-based ELISA for quantitation of human plasma Lp(a) was developed and compared with existing assays to validate its accuracy.
Does the new monoclonal antibody-based ELISA accurately quantify Lp(a) in human plasma compared to existing methods?
Does the new monoclonal antibody-based ELISA accurately quantify Lp(a) in human plasma compared to existing methods?
The developed monoclonal antibody-based ELISA provides a reliable method for quantifying Lp(a) in human plasma, comparable to existing assays.
Supports assay comparability in preclinical work; leaves open clinical adoption of mAb 2F4E7 ELISA pending human validation.
The present study describes a monoclonal antibody-based enzyme immunoassay (ELISA) for the quantitation of lipoprotein(a), Lp(a), in human plasma. Two antibodies to Lp(a), 2F4E7 and 8G12G7, were produced and characterized as specific and high affinity antibodies against Lp(a). A reference control serum was utilized to prepare the standard curve in a Lp(a) concentration range from 0.015 to 0.5 ug/ml. A biotinylated monoclonal antibody against apoB-LDL was used as the second antibody. The comparison of the standardized ELISA using mAb 2F4E7 with an ELISA using a characterized mAb against Lp(a) (clone KO9) as capture antibody showed that the Lp(a) concentration of two standard sera was similar with both assays. Furthermore, when compared with an electroimmunoassay kit, similar Lp(a) concentrations for the standard were also obtained.
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Bustos et al. (2002) studied Lipoprotein(a) quantitation. Monoclonal antibody-based ELISA using mAb 2F4E7 vs. ELISA using clone KO9 and electroimmunoassay kit was evaluated on Lp(a) concentration of standard sera. A novel monoclonal antibody-based ELISA using mAb 2F4E7 for human Lp(a) quantitation yielded similar concentrations to an established ELISA and an electroimmunoassay kit.
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