What is the clinical evidence from this study?

Study design: Other. Intervention: asp1+ gene disruption vs. Wild-type or non-disrupted strains. Primary outcome: Morphology, growth properties, and genetic interactions.

What question did this study set out to answer?

To identify and characterize proteins that functionally interact with the Arp2/3 complex and actin in the fission yeast Schizosaccharomyces pombe.

July 1, 1999Genetics

Identification and Characterization of Schizosaccharomyces pombe asp1+, a Gene That Interacts with Mutations in the Arp2/3 Complex and Actin

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Key result

Disruption of the asp1+ gene in Schizosaccharomyces pombe leads to altered morphology, hypersensitivity to Ca+ and low pH, and synthetic lethality with Arp2/3 complex mutations.

Population

Schizosaccharomyces pombe (fission yeast) and Saccharomyces cerevisiae strains

Design

Preclinical

Authors

AFAnna FeoktistovaVanderbilt University DMDannel McCollumUniversity of Massachusetts Chan Medical School RORyoma OhiUniversity of Michigan

Discussion

Member takes

Implication

Does not alter clinical practice; leaves open whether ASP1 homologs influence cardiac ion handling or cytoskeletal dynamics.

Key Points

To identify and characterize proteins that functionally interact with the Arp2/3 complex and actin in the fission yeast Schizosaccharomyces pombe.
Screened for high-copy suppressors of the temperature-sensitive S. pombe arp3-c1 mutant to isolate interacting genes.
Generated and phenotyped asp1 gene disruption strains under heat stress, low pH, and elevated calcium conditions.
Assessed synthetic lethal interactions by crossing asp1 disruption mutants with Arp2/3 complex mutants (arp3-c1, sop2-1) and an actin mutant (act1-48), alongside orthologous tests in Saccharomyces cerevisiae.
Identified asp1+, which encodes a conserved 106-kD protein (921 amino acids) whose loss causes defects in polarized growth, altered morphology, and hypersensitivity to high temperatures, low pH, and Ca²⁺ ions.
Found that asp1 disruption causes synthetic lethality when combined with arp3-c1, sop2-1, or act1-48 mutations.
Observed a negative genetic interaction between the S. cerevisiae ortholog vip1 disruption strain and a las17Δ strain, confirming a conserved role in the cortical actin cytoskeleton.

Structured PICO

Population

Schizosaccharomyces pombe (fission yeast) and Saccharomyces cerevisiae strains

Intervention

Disruption of asp1+ (or vip1 in S. cerevisiae) and genetic interactions with Arp2/3 complex mutations

Outcome

Phenotypic effects of asp1+ disruption (morphology, growth, sensitivity to Ca+ and low pH) and genetic interactions (synthetic lethality with arp3-c1, sop2-1, and act1-48)

Asp1p/Vip1p is identified as an important protein for the function of the cortical actin cytoskeleton in yeast.

Cite This Study

Feoktistova et al. (1999) studied this question. asp1+ gene disruption vs. Wild-type or non-disrupted strains was evaluated on Morphology, growth properties, and genetic interactions. Disruption of the asp1+ gene in Schizosaccharomyces pombe leads to altered morphology, hypersensitivity to Ca+ and low pH, and synthetic lethality with Arp2/3 complex mutations.

synapsesocial.com/papers/6ab32cb6ccaa2222efcb5987 https://doi.org/10.1093/genetics/152.3.895

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Identification and Characterization of Schizosaccharomyces pombe asp1+, a Gene That Interacts with Mutations in the Arp2/3 Complex and Actin

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Identification and Characterization of Schizosaccharomyces pombe asp1+, a Gene That Interacts with Mutations in the Arp2/3 Complex and Actin

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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