Rambouillet wool fiber was scoured and treated with transglutaminase (TG) enzyme for different concentrations and duration at neutral pH at 45°C. The physical, mechanical, dyeing, and antimicrobial properties of the wool fiber before and after enzymatic treatment were evaluated as per standard methods. Results inferred that TG enzyme-treated wool fiber sample showed improvement in softness, luster, and tensile strength in comparison with scoured wool fiber. It is found that 0.5% TG enzyme treatment at 45°C for 60 min at pH 7 was an optimum condition. Scanning electron microscope images also confirmed the flattened and smooth scales of wool fiber after enzymatic treatment.
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