Key result
RT-PCR and RT-LAMP assays successfully detected Foot and Mouth Disease Virus, showing 100% positive results in ELISA-confirmed samples from the 2017 outbreaks in Punjab.
RT-PCR and RT-LAMP assays successfully detected FMDV in ELISA-confirmed and field samples from livestock in Punjab.
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RT-LAMP may enable rapid FMDV field detection in outbreaks; leaves open prospective validation before routine veterinary adoption.
Foot and mouth disease is infectious, tranboundary, disease of cloven hoofed domesticated ruminants. In this study, ELISA confirmed positive infected and field samples were analyzed through RT-PCR using universal primer set (IF/IR and P1/P2) and RT-LAMP assays. A total of N=100 samples were collected from different livestock population areas in Punjab. Types of samples were serum, nasal secretions, tissue, saliva and swab samples. Out of N=100 infected samples, n=19 samples were serotyped into A, O and Asia 1 strains through antigen ELISA (Ag- ELISA) and these samples were further confirmed through RT-PCR and RT-LAMP assays. ELISA confirmed samples revealed 100% results with RT-PCR and RT-LAMP assays. Field samples revealed 33.33%, 27.16% and 37.03% with RT-PCR (IF/IR and P1/P2) and RT-LAMP assays respectively. It was concluded that ELISA confirmed samples were successfully analyzed with RT-PCR and RT-LAMP assays. Both the assays successfully analyzed 2017 outbreaks of FMD in Punjab.
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Haidar et al. (2018) studied Foot and mouth disease (n=100). RT-PCR and RT-LAMP assays vs. ELISA was evaluated on Detection of FMDV. RT-PCR and RT-LAMP assays successfully detected Foot and Mouth Disease Virus, showing 100% positive results in ELISA-confirmed samples from the 2017 outbreaks in Punjab.
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