Key result
Free flow isotachophoresis isolates 4 distinct apoB subfractions differing in lipid composition and cell-binding affinity.
Why the study?
The characterization of apoB-containing lipoproteins separated by preparative free flow isotachophoresis from fasting and postprandial sera of normolipidemic individuals was investigated to understand their composition and cell binding properties.
Population
Fasting and postprandial sera from normolipidemic individuals
Comparison
ApoB-containing lipoproteins from fasting vs postprandial sera
Design
Preclinical study using preparative free flow isotachophoresis
Authors
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Supports functional apoB lipoprotein subclassification in animals; leaves open human relevance and should not yet change practice.
Preparative free flow isotachophoresis can separate apoB-containing lipoproteins into distinct functional subclasses with different receptor binding affinities.
Nowicka et al. (1990) studied Normolipidemic. Preparative free flow isotachophoresis (ITP) vs. Fasting vs postprandial sera was evaluated on Lipoprotein fractionation and binding affinity to fibroblasts and HepG2 cells. Preparative free flow isotachophoresis separated apoB-containing lipoproteins into four major groups with distinct lipid compositions and binding affinities to fibroblasts and HepG2 cells.
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