Key result
Swapping specific sub-regions of wild-type and optimized Ago sequence identified the portion of the coding region (nucleotides 1-1163 for Ago-3 and 1-1494 for Ago-4) most influential for expression.
Population
Mammalian argonaute proteins (Ago1-Ago4) coding sequences
Comparison
Optimization of Ago3 and Ago4 coding sequences vs Wild-type Ago3 and Ago4 sequences
Design
Preclinical
Authors
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Codon optimization rescues Ago3/Ago4 expression for functional assays; leaves open their redundant roles in mammalian gene silencing.
Specific sequence features in the coding regions of Ago3 and Ago4 limit their expression levels in mammalian tissues.
Valdmanis et al. (2011) studied this question. Swapping specific sub-regions of wild-type and optimized Ago sequence vs. wild-type sequence was evaluated on expression levels of Ago3 and Ago4. Swapping specific sub-regions of wild-type and optimized Ago sequence identified the portion of the coding region (nucleotides 1-1163 for Ago-3 and 1-1494 for Ago-4) most influential for expression.
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