Key result
Gene expression in adipose tissue CD14+ cells of obese T2DM patients was significantly different from obese non-T2DM patients, with 19 up-regulated genes and TNF identified as a core protein node.
Observational
Bioinformatics analysis reveals that TNF and specific microRNAs play a key role in the altered gene expression of adipose tissue CD14+ cells in obese T2DM patients.
Highlights TNF as potential target in obese T2DM inflammation; hypothesis-generating and requires validation before clinical consideration.
OBJECTIVES: cells in patients with Type 2 diabetes mellitus (T2DM) based on chip data, screen differentially expressed genes, and analyze their relationship with the environmental factors. METHODS: The data of GSE54350 were obtained from the public database of gene expression profiling. The data were pre-processed by Network Analyst, String 11.0, Cytoscape 3.7.1, and other analytical software. The differentially expressed genes were analyzed by gene ontology biological function and kyoto encycopedia of genes and genomes (KEGG) signaling pathway to establish differential gene protein interaction network, transcription factor-gene regulatory network, microRNA-gene regulatory network, environmental factors-gene regulatory network, and other interaction systems. RESULTS: cells in adipose tissue of obese T2DM patients was significantly different from that of obese non-T2DM patients. There were 19 differentially expressed genes with up-regulation. The differentially expressed genes were mainly involved in ARP2/3 complex regulation of actin cytoskeleton, positively associated with biological processes such as protein complex assembly, and involved in the phagocytic Fcγ receptor signaling pathways and receptor family signaling pathways. The protein-protein interaction networks showed that TNF was the core protein node. The microRNA-gene regulatory network showed that hsa-mir-124-3p interacted with differentially expressed genes; TNF, KYNU, RCAN1 and other related genes all interacted with environmental factors. CONCLUSIONS: cells are significantly changed in obese T2DM patients. TNF may play an important role in the process of obesity affecting the immune status of T2DM patients. Multiple microRNAs, transcription factors, and environmental factors also play a role in the above process. This study provides new material and new ideas for further exploration of the impact of obesity on T2DM patients.
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Duan et al. (2021) conducted an observational in Type 2 diabetes mellitus and obesity. Type 2 diabetes mellitus vs. Obese non-T2DM patients was evaluated on Differentially expressed genes in adipose tissue CD14+ cells. Gene expression in adipose tissue CD14+ cells of obese T2DM patients was significantly different from obese non-T2DM patients, with 19 up-regulated genes and TNF identified as a core protein node.
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