Effect of protein–protein interactions on protein aggregation kinetics

The mutual diffusion coefficient Dm of dilute lysozyme solutions is measured by the dynamic light scattering technique. The relation between the osmotic second virial coefficient B22 and the protein mutual diffusion coefficient Dm of dilute solutions is examined experimentally and theoretically. The role of B22 in relation with Dm in protein aggregation kinetics was analyzed based on the effect of the average protein pairwise interactions on the protein aggregation kinetics. The empirical “crystallization window” proposed by George and Wilson [Acta Crystallogr., Sect. D: Biol. Crystallogr. 50, 361 (1994)] can be interpreted from the point view of crystallization kink kinetics.