Key result
tBuBHQ inhibits cardiac and skeletal SR Ca2+-ATPase activity by up to ~90%.
Why the study?
The interaction and inhibitory effects of 2,5-di(tert-butyl)-1,4-benzohydroquinone on sarcoplasmic reticulum Ca(2+)-ATPase isoforms from skeletal and cardiac muscles were characterized.
Population
SR Ca(2+)-ATPase from rabbit fast-twitch skeletal and canine cardiac muscles
Comparison
2,5-di(tert-butyl)-1,4-benzohydroquinone vs control
Design
Preclinical biochemical study
Authors
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tBuBHQ offers a probe for SR Ca2+-ATPase isoforms; extends biochemical tools but leaves open cardiac applications.
tBuBHQ is a potent inhibitor of the sarcoplasmic reticulum Ca(2+)-ATPase in both skeletal and cardiac muscle, acting at multiple sites in the reaction sequence.
Nakamura et al. (1992) studied this question. 2,5-di(tert-butyl)-1,4-benzohydroquinone (tBuBHQ) vs. control was evaluated on ATP hydrolysis by SR Ca(2+)-ATPase. 2,5-di(tert-butyl)-1,4-benzohydroquinone (tBuBHQ) potently inhibited ATP hydrolysis by fast-twitch skeletal and slow-twitch skeletal/cardiac isoforms of SR Ca2+-ATPase by up to 80-90%.
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