Isolation and Characterization of Melanoidines From Malt and Malt Roots

Preparative isolation of alcohol-soluble and water-soluble melanoidines from pale malt, caramel malt, coffee malt and malt roots was done. The distribution of melanoidines according to molecular weight and the spectral characterization of the separate fractions was studied by gel chromatography on Sephadex G-50. The melanoidines isolated from coffee malt have the largest molecular weights and those from pale malt the smallest. The alcohol-soluble melanoidines of malt are characterized by a greater diversity in spectra and absorbance maxima from 240 to 285 nm. The water-soluble melanoidines absorb in the region 265 to 283 nm.