Key result
A 1552-base cDNA sequence for the zebrafish muscle-specific isoform of creatine kinase was isolated, encoding a 381 amino acid protein similar to those in other species.
The study successfully isolated and characterized the cDNA sequence for the zebrafish muscle-specific creatine kinase, providing a basis for further molecular studies.
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No immediate clinical impact; extends creatine kinase sequence data for future zebrafish model studies.
We have isolated and characterized a cDNA sequence corresponding to the zebrafish muscle-specific isoform of creatine kinase. The sequence is 1552 bases in length and contains an open reading frame capable of producing a 381 amino acid protein. The sequence is very similar to muscle-specific creatine kinases isolated from other species at both the nucleotide and amino acid levels but contains some differences from a previously reported zebrafish clone.
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Harder et al. (2001) studied this question. A 1552-base cDNA sequence for the zebrafish muscle-specific isoform of creatine kinase was isolated, encoding a 381 amino acid protein similar to those in other species.
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