Isolation, Identification and Antibiogram of Mannheimia hemolytica Associated with Caprine Pneumonia in the Cauvery Delta Region of Tamil Nadu, India

A field investigation was made in an organized goat farm near thanjavur city with the history of sudden mortality due to respiratory distress. About 300 tellicherry adult goats are maintained in that farm. Bilateral serous to mucopurulent nasal discharge, lymphadenopathy and respiratory stridor were observed in few animals. Samples such as nasal swab, trans tracheal lavage, blood samples, fecal samples and lymph node aspiration smear were collected from affected animals aseptically for microbiological and parasitiological examination. Nasal swabs and tracheal lavages were analyzed for bacteriological examination using standard methods. Manheimia haemolytica could be isolated and confirmed by multiplex PCR using hypothetical protein, Lkt and Lkt2 of leukotoxin D gene and 16S rDNA gene primers. The isolate was found to be susceptible to Cetriaxone, Amikacin and Enrofloxacin while the isolate was resistant to Tetracycline, Gentamicin and Streptomycin. The fecal samples, blood smear and lymph node aspiration smear were negative for parasitic ova, cyst and blood protozoan. The isolation and identification of M. haemolytica reflect the possible role causing respiratory disease encountered in goat flocks.