Key result
A sensitive and specific LC-MS/MS method was developed and validated for quantifying Rucaparib in human plasma, demonstrating high precision and accuracy over a range of 5.0-5000.0 pg/mL.
A sensitive and specific LC-MS/MS method was successfully developed and validated for the quantification of Rucaparib in human plasma.
May aid rucaparib pharmacokinetic research; leaves open clinical validation for therapeutic monitoring.
A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of Rucaparib (RP) in human plasma using Rucaparib-d3 (RPD3) as an internal standard (IS).Chromatographic separation was performed on Xbridge C18, 50 x 4.6 mm, 5 m column with an isocratic mobile phase composed of 10mM Ammonium formate and Methanol in the ratio of (20:80 v/v), at a flow-rate of 0.7 mL/min.RP and RPD3 were detected with proton adducts at m/z 323.4 170.1 and 328.4 170.1 in multiple reaction monitoring (MRM) positive mode respectively.Liquid-Liquid extraction method was used to extract the drug and IS.The method was validated over a linear concentration range of 5.0-5000.0pg/mL with correlation coefficient (r 2 ) ≥ 0.9994.This method demonstrated intra and inter-day Precision within 0.7 to 2.0 and 0.7 to 2.7 % and Accuracy within 101.4 to 102.4 and 99.5 to 104.8 %.Rucaparib (RP) was found to be stable throughout freezethawing cycles, bench top and postoperative stability studies.
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Vamseekrishna Gorijavolu (2018) studied this question. LC-MS/MS method for Rucaparib quantification was evaluated on Method validation (linearity, precision, accuracy). A sensitive and specific LC-MS/MS method was developed and validated for quantifying Rucaparib in human plasma, demonstrating high precision and accuracy over a range of 5.0-5000.0 pg/mL.
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