Key result
A low-cost, disposable point-of-care testing device employing LAMP successfully detected MRSA genomic DNA and foot-and-mouth disease virus with a time to result of ~90 minutes.
A novel, low-cost, disposable point-of-care device using LAMP technology can successfully detect pathogens like MRSA and foot-and-mouth disease virus in approximately 90 minutes.
May support low-cost POC pathogen detection development; leaves open human clinical validation and utility.
Development of small footprint, disposable, fast, and inexpensive devices for pathogen detection in the field and clinic would benefit human and veterinary medicine by allowing evidence-based responses to future out breaks. We designed and tested an integrated nucleic acid extraction and amplification device employing a loop-mediated isothermal amplification (LAMP) or reverse transcriptase-LAMP assay. Our system provides a screening tool with polymerase-chain-reaction-level sensitivity and specificity for outbreak detection, response, and recovery. Time to result is ∼90 min. The device utilizes a swab that collects sample and then transfers it to a disc of cellulose-based nucleic acid binding paper. The disc is positioned within a disposable containment tube with a manual loading port. In order to test for the presence of target pathogens, LAMP reagents are loaded through the tube's port into contact with the sample containing cellulose disc. The reagents then are isothermally heated to 63°C for ∼1 h to achieve sequence-specific target nucleic acid amplification. Due to the presence of a colorimetric dye, amplification induces visible color change in the reagents from purple to blue. As initial demonstrations, we detected methicillin resistant Staphylococcus aureus genomic DNA, as well as recombinant and live foot-and-mouth disease virus.
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Bearinger et al. (2010) studied Pathogen detection. Integrated nucleic acid extraction and amplification device employing LAMP or RT-LAMP was evaluated on Detection of target pathogens. A low-cost, disposable point-of-care testing device employing LAMP successfully detected MRSA genomic DNA and foot-and-mouth disease virus with a time to result of ~90 minutes.
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