The effect of pasteurization on the microbiological and sensory quality of white grape juice and wine was investigated. Heat treatment of less than 3 Pasteurization Units (PUs = minutes at 60°C or equivalent) was sufficient to reduce 106 cells/mL of Saccharomyces cerevisiae and Hansenula anomala added to Chenin blanc juice to an undetectable level. In contrast, heat treatment exceeding 105 PUs was required to have a measurable browning effect on juice, as measured by absorbance at 420 nm and by formation of 5-hydroxymethyl-furfural. Furthermore, up to 50,000 PUs did not significantly affect flavor, as determined by duo-trio tests with 18 trained judges. Pasteurized (23 PUs) and nonpasteurized juice fermented at the same rate, although not as rapidly as pasteurized, thiamine-supplemented juice. Tunnel pasteurization (13.7 PUs) reduced 5 × 106 yeast cells/mL added to grape juice to an undetectable level and did not significantly affect juice or wine flavor.
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MALLETROIT et al. (1991) studied this question.
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