Key result
Resveratrol pretreatment significantly attenuated reactive oxygen species production and prevented cellular senescence in H2O2-induced premature aging of vascular endothelial cells.
Why the study?
Does resveratrol prevent oxidative stress-induced senescence in cultured human umbilical vein endothelial cells?
Does resveratrol prevent oxidative stress-induced senescence in cultured human umbilical vein endothelial cells?
p-value: p=<0.05
Resveratrol demonstrates exercise-mimetic effects in vitro by protecting vascular endothelial cells against oxidative stress-induced premature senescence and preserving angiogenic function.
Resveratrol merits further preclinical vascular aging studies; leaves open clinical translation in humans.
PURPOSE: Regular exercise has been shown to have anti-aging effects on the vascular system by improving endothelial function and diminishing oxidative stress. However, adherence to an exercise regimen can be difficult for some individuals, particularly those with limited mobility. This study investigated the effects of resveratrol as an exercise mimetic for preventing and improving oxidative stressinduced senescence in vascular endothelial cells.METHODS: Cultured human umbilical vein endothelial cells were used to investigate the reversal effects of laminar shear stress and the protective effects of resveratrol on oxidative stress-induced premature senescence. Senescence was estimated using SA-β-gal staining and western blot analysis, whereas oxidative stress levels were evaluated using MitoSOX staining. Matrigel tube formation and scratch assays were also performed to assess endothelial angiogenic and migration functions.RESULTS: The study showed that laminar shear stress could partly normalize premature endothelial senescence. Resveratrol pretreatment attenuated reactive oxygen species production and prevented senescence in H2O2-induced premature aging. Additionally, resveratrol contributed to maintaining endothelial cells’ angiogenic and migratory functions.CONCLUSIONS: This study demonstrated that laminar shear stress can mitigate the effects of oxidative stress-induced premature senescence. Resveratrol may be a potential substitute for exercise to prevent age-related damage to the endothelium by increasing resistance to oxidative stress and improving endothelial function.
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Kim et al. (2023) studied Vascular endothelial senescence. Resveratrol vs. H2O2 alone or non-treated control was evaluated on Proportion of Senescence-Associated β-Galactosidase (SA-β-gal) positive cells (p=<0.05). Resveratrol pretreatment significantly attenuated reactive oxygen species production and prevented cellular senescence in H2O2-induced premature aging of vascular endothelial cells.
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