Improvement of Amperometric Biosensor Performance for H 2 O 2 Detection based on Bimetallic PtM (M = Ru, Au, and Ir) Nanoparticles

Novel bimetallic nanoparticles have been synthesized via rapid microwave irradiation, leading to an improved sensitivity and a highly anti‐interference property for amperometric biosensor in H 2 O 2 detection. The material characterizations were performed by TEM, XRD, and EDX, which show the bimetallic formation of Pt‐based catalysts and well‐dispersed nanoparticles of 2–5 nm. The sensitivities for the detection of H 2 O 2 of PtRu, PtAu, and PtIr as the biosensor working electrode catalysts are 539.01 ( R 2 = 0.99), 415.46 ( R 2 = 0.99), and 404.52 ( R 2 = 0.97) μA mM −1 cm -2 , respectively, nearly twice higher than the pure Pt catalyst (221.77 μA mM −1 cm −2 , R 2 = 0.98), at a low applied potential of +0.25 V versus Ag/AgCl. Furthermore, Pt‐Ru and Pt‐Ir show a highly sensitive response and a promising anti‐interference capability to ascorbic acid, a major interferent, by reducing the interferent current as low as 7‐8% significantly lower than that of Pt (30% change). The enhancement of both sensitivity and selectivity in the bimetallic catalysts can be found practical applications in biosensing.