Key result
Ultrafiltration combined with Ficoll gradient centrifugation provided substantially better recovery of infectivity and virion RNA-dependent DNA polymerase activity than conventional methods.
SUMMARY The Amicon TCIR recycle system with Diaflo PM30 filters provided a rapid, simple and inexpensive method for the concentration of baboon endogenous virus from a large quantity of culture medium from chronically infected cells. The recovery of infectivity and virion RNA-dependent DNA polymerase activity was substantially better than after the conventional concentration methods of ultra-centrifugation or precipitation with ammonium sulphate or polyethylene glycol 6000. The combination of ultrafiltration and Ficoll gradient centrifugation is recommended as a general procedure for the purification of fragile C-type viruses.
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Tamura et al. (1978) studied Baboon endogenous virus from chronically infected cells. Ultrafiltration by Diaflo membrane and purification by Ficoll gradient centrifugation vs. Ultra-centrifugation or precipitation with ammonium sulphate or polyethylene glycol 6000 was evaluated on Recovery of infectivity and virion RNA-dependent DNA polymerase activity. Ultrafiltration combined with Ficoll gradient centrifugation provided substantially better recovery of infectivity and virion RNA-dependent DNA polymerase activity than conventional methods.
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