January 1, 1978Intervirology
A Simple Method for Concentration of Enteroviruses and Rotaviruses from Cell Culture Harvests Using Membrane Filters
Structured PICO
P
Population
Cell culture harvests containing enteroviruses and rotaviruses
I
Intervention
Blending cell culture harvests with fluorocarbon, followed by adsorption to epoxy-fiberglass filters in acidified harvests, and elution with high pH buffer
O
Outcome
Virus recovery and concentrationsurrogate
Blending cell culture harvests with fluorocarbon removes membrane-coating components, allowing for efficient concentration of enteroviruses and rotaviruses using membrane filters.
Authors
Discussion
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Implication
May advance lab virus concentration methods; leaves open clinical translation as Level 5 animal data.
Cite This Study
Farrah et al. (1978) studied this question.