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Use of ethylenediaminetetraacetate (EDTA) as the chelating agent in the biuret reaction (in place of tartrate or citrate) was investigated. We found that this suggested modification of the reagent results in lower color yields and prolongs the time for color development. The EDTA reagent is more stable if color development is hastened by heating, but conditions are described under which the more customary reagent may be so used.
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Kley et al. (1973) studied this question.
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