Key result
Renal distribution of CNP and ANPR-B mRNA suggests local autocrine and paracrine signaling mechanisms.
Why the study?
The presence and localization of C-type natriuretic peptide and its B-type receptor mRNAs in rat renal structures were not fully characterized.
CNP can be produced locally in the glomerulus and renal vascular system and may act in autocrine and paracrine fashions to influence renal function.
No clinical implications yet; leaves open local CNP signaling roles in rat kidney for mechanistic follow-up.
The present study was undertaken to investigate the presence of C-type natriuretic peptide (CNP) mRNA and its receptor, natriuretic peptide B-type receptor (ANPR-B) mRNA, in rat renal structures. The microlocalization of mRNAs coding for CNP and ANPR-B was carried out in the rat kidney, using an assay of reverse transcription and polymerase chain reaction (RT-PCR) in individual microdissected renal tubule segments, glomeruli, vasa recta bundle, and arcuate arteries. The PCR signal for CNP was detected in glomerulus, vasa recta bundle, and arcuate artery. The PCR product of ANPR-B was widely present in renal structures. Relatively large amounts of ANPR-B PCR product were detected in glomerulus, vasa recta bundle, arcuate artery, and distal nephron segments. A relatively high concentration of CNP (10(-7) M) stimulated guanosine 3',5'-cyclic monophosphate accumulation in glomerulus, medullary thick ascending limb, cortical collecting duct, and inner medullary collecting duct. Our data demonstrate that CNP can be produced locally in the glomerulus and renal vascular system and that ANPR-B is widely distributed in renal structures. Thus CNP may influence renal function and act in autocrine and paracrine fashions in the kidney.
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Terada et al. (1994) studied this question. C-type natriuretic peptide (CNP) was evaluated on Localization of mRNAs coding for CNP and ANPR-B. CNP mRNA is localized in the glomerulus and renal vascular system, while ANPR-B mRNA is widely distributed in renal structures, suggesting CNP acts in autocrine and paracrine fashions in the kidney.
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