Murine norovirus infection in Brazilian animal facilities

Figures

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  Fig. 1.. RAW 264.7 cells infected with MNV. Original magnification ×400. (A) Uninfected RAW264.7 cells. (B) and (C): RAW264.7 cells infected with a fecal sample from a B6.129S7-Rag1tm1Mom mouse. CPE at 48 h post-infection, showing a typical vesiculated (*) and rounded cell.

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  Fig. 2.. Nucleotide sequence alignment of the capsid gene (VP1) sequences of MNV. LCQS1 corresponds to the sequence isolated in cell culture; LCQS2, LCQS3, LCQS4, LCQS5, LCQS6, LCQS7, LCQS10, and LCQS11 are sequences derived from PCR products of naturally infected mice. MNV reference strains are represented by GenBank accession numbers. Conserved nucleotides are shaded in gray, and variable regions are represented by coloured nucleotides.

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  Fig. 3.. Phylogenetic analysis based on the nucleotide sequences of the viral protein (VP1) genes from MNV isolates and strains of genogroup V. Norwalk virus (M87661) and Sapporo virus (X86560) sequences were used as outgroups. LCQS1 refers to the MNV isolate from infected RAW 264.7 cells. LCQS2, LCQS3, LCQS4, LCQS5, LCQS6, LCQS7, LCQS10, and LCQS11 refer to fecal samples of naturally infected mice from southeast animal facilities. A phylogenetic tree was constructed with MEGA6 software using the Neighbor-Joining method and evolutionary distances was computed using the Kimura-2 model and bootstrap values defined for 1,000 replicates. The scale bar is units per nucleotide substitution. Brazilian MNV sequences are identified by symbols ▲ and ♦.