Key result
Palladium-mediated bioorthogonal labeling enables second-scale temporal imaging of live enterovirus-71 cell entry.
Why the study?
Methods for efficient labeling of live non-enveloped viruses and tracing virus entry processes remain limited.
Population
Live enterovirus-71 (EV71) virions and live host cells
Comparison
Site-specific bioorthogonal labeling with organometallic palladium reagent vs immunofluorescence and membrane-anchored dyes
Design
Preclinical study using site-specific chemical labeling
Authors
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Site-specific Pd-mediated labeling enables real-time EV71 entry imaging in live cells; extends cysteine bioconjugation to non-enveloped viruses for virology studies.
A novel organometallic palladium reagent enables highly efficient, site-specific fluorescent labeling of live non-enveloped viruses like EV71 to monitor viral entry dynamics.
Wang et al. (2018) studied Enterovirus-71 (EV71) infection. Site-specific bioorthogonal labeling mediated by an organometallic palladium reagent vs. Immunofluorescence and membrane-anchored dyes was evaluated on Visualization of virus entry into live cells. Site-specific bioorthogonal labeling using an organometallic palladium reagent allowed temporal imaging of live enterovirus-71 entry into host cell membranes on the timescale of seconds.
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