Key result
An imaging-based approach utilizing FRET resolved by FLIM with a specific plasmid construct successfully detected enterovirus 71 infection in HeLa cells through increased GFP2 fluorescence lifetime.
Population
HeLa cells expressing a plasmid construct with GFP2 and DsRed2 linked by a 12-amino-acid-long cleavage…
Design
Preclinical
Authors
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May enable specific in vitro detection of enterovirus 71; leaves open translation to clinical diagnostics or in vivo models.
FRET resolved by FLIM offers a novel, imaging-based approach for the specific detection of enterovirus 71 infection in cells.
Ghukasyan et al. (2007) studied Enterovirus 71 (EV71) infection. Fluorescence resonance energy transfer (FRET) resolved by fluorescence lifetime imaging microscopy (FLIM) was evaluated on Detection of EV71 infection via increased GFP2 fluorescence lifetime. An imaging-based approach utilizing FRET resolved by FLIM with a specific plasmid construct successfully detected enterovirus 71 infection in HeLa cells through increased GFP2 fluorescence lifetime.
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