Key result
The SAT-EV71 method with an internal control detected EV71 VP1 with a minimum of 10 copies per reaction, demonstrating high sensitivity and specificity while preventing false negatives.
Why the study?
Does the SAT-EV71 method with internal control accurately detect EV71 in clinical specimens?
Does the SAT-EV71 method with internal control accurately detect EV71 in clinical specimens?
The SAT-EV71 assay with an internal control provides a sensitive and specific method for the molecular diagnosis of EV71 in suspected cases of hand, foot, and mouth disease.
May support SAT-EV71 evaluation for EV71 detection; leaves open prospective clinical validation before routine use.
Human enterovirus 71 (EV71) is a primary etiological agent of hand, foot, and mouth disease (HFMD). The associated infections have posed a serious threat to the public health. In the present study, a real-time simultaneous amplification and testing (SAT) technology was developed for detecting EV71 (SAT-EV71). The RNA of EV71 and an internal control (IC) were amplified and analyzed simultaneously by isothermal amplification and real-time detection of fluorescence using routine real-time PCR. Furthermore, this SAT-EV71 method with IC was evaluated by analyzing 256 clinical specimens including 60 enterovirus-positive ones confirmed by the virus-cell culture method. The other 196 putative ones were further analyzed by a PCR-fluorescence probing assay. The results showed that SAT-EV71 can detect the EV71 VP1 with a minimum of 10 copies per reaction at an optimal concentration of IC (5000 copies per reaction) with a high sensitivity and specificity. Meanwhile, the use of IC can prevent false negatives effectively by monitoring the processes of nucleic acid extraction and amplification. Overall, this easy-to-perform SAT-EV71 with IC can detect EV71 sensitively and specifically. It might be used in the molecular diagnosis of EV71 in putative cases of HFMD.
No takes yet. Share an insight, caveat, or question.
Chen et al. (2013) studied Enterovirus 71 (EV71) infection / Hand, foot, and mouth disease (n=256). SAT-EV71 (simultaneous amplification and testing technology) vs. Virus-cell culture method and PCR-fluorescence probing assay was evaluated on Detection of EV71 VP1. The SAT-EV71 method with an internal control detected EV71 VP1 with a minimum of 10 copies per reaction, demonstrating high sensitivity and specificity while preventing false negatives.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: