Key result
45 nucleotides of the 3' end of the M1 coding region are sufficient for efficient expression of the downstream protein from the stop-start pentanucleotide in influenza B virus.
A specific 45-nucleotide region from the influenza B virus M gene can be used to efficiently express multiple proteins from a single mRNA.
Minimal M1 sequence suffices for BM2 expression; extends viral translation models but leaves open therapeutic targeting.
Segment 7 of influenza B virus encodes two proteins, M1 and BM2. BM2 is expressed from a stop-start pentanucleotide, in which the BM2 initiation codon overlaps with the M1 stop codon. Here, we demonstrate that 45 nucleotides of the 3' end of the M1 coding region, but not the 5' end of the BM2 coding region, are sufficient for the efficient expression of the downstream protein. Placing these 45 nucleotides and the stop-start pentanucleotide in between the coding sequences induced the expression of at least three noninfluenza proteins, suggesting the utility of this system for expressing multiple proteins from one mRNA.
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Hatta et al. (2009) studied Influenza B virus. 45 nucleotides of the 3' end of the M1 coding region was evaluated on Protein expression from the stop-start pentanucleotide. 45 nucleotides of the 3' end of the M1 coding region are sufficient for efficient expression of the downstream protein from the stop-start pentanucleotide in influenza B virus.
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