Role of Single Nucleotide Polymorphism of Matrix Metalloproteinase (MMP-13 rs2252070) in the Etiology of Dental Caries: A PCR Based Study

Role Of Single Nucleotide Polymorphism Of Matrix Metalloproteinase (MMP-13 rs2252070) In The Etiology Of Dental Caries: A PCR Based Study.Background: Dental caries is multifactorial in origin.This study provides an alternative hypothesis for causation of dental caries and emphasize that genetics has a major role in disease process.Methods: The subjects were divided into 4 groups based on oral hygiene and dental caries experience.Polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was used in this study.Blood samples (2 ml) were obtained from the patients followed by isolation of Genomic DNA, Polymerase chain reaction test and digestion with enzyme Bsr-I. Results:The size of the initial PCR products was 450bp.After digestion with restriction enzyme Bsr-I the 450bp products were completely digested with two restriction sites (250 &180) in a group of subjects with good oral hygiene and presence of dental caries.Other groups had incomplete digestion With Bsr-I.Conclusion: Due to the single nucleotide polymorphism of MMP-13(rs2252070) the patients were suffering from dental caries despite having good oral hygiene.Genes play a major role in progression of dental caries. Practical implication:The established relation will help in formulating guidelines for prevention and treatment of cariogenic subjects more effectively.The pattern of dental caries can be learned in more precise manner.