Assessment of Microrna-182 and Microrna-133 as Non-Invasive Predictors of Breast Cancer in Pakistan

Background: Breast cancer is a diverse heterogeneous disease and it has become difficult to track the progression of breast cancer. It has been reported that miRNAs play a role in therapeutic targets and diagnostic markers. Methods: In the study, the expression levels of miRNA-182, and miRNA-133 were assessed of total 292 patients through purposive sampling in one of the public hospitals of Pakistan. Out of which 165 were cases and 127 were controls. Results: The results of the study revealed that in both groups the levels of miRNA-182 and miRNA-133 expression levels were assessed. Hence the expression levels of miRNA-182, in both groups were similar in terms of age, status of menopause, and BMI. The patients suffering from breast cancer expressed more miRNA-133 than the control group did, and there were notable differences between the two groups when it came to older age, status of menopause, and BMI (obesity). A sensitivity of 94.1%, specificity of 100%, positive predictive value of 100 %, and negative predictive value of 80.81% were obtained for miRNA-133 levels. These findings suggested that miRNA-133 possessed strong predictive power. A sensitivity of 74.52%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 59.38% were obtained for miRNA-182. Conclusion: The study concludes that the function of miRNA-182 and miRNA-133 are considered As Non-Invasive Predictors in the course and outcome of Breast Cancer.