Key result
Bovine preproendothelin-2 cDNA encodes a 177-amino acid polypeptide expressed in various organs including the heart, lung, liver, kidney, and gastrointestinal tract, but not the spleen.
The study successfully cloned bovine preproendothelin-2 cDNA and mapped its transcript distribution across various organs, including ruminant-specific digestive organs.
Bovine PPET2 sequence now available; leaves open any cardiovascular role pending functional and translational studies.
Endothelin-2 (ET2), which was originally identified in human, is a bioactive peptide of 21 amino acids with strong vasoconstrictive and pressor effects. Here we report the cDNA cloning and characterization of bovine preproendothelin-2 (PPET2), the precursor form of ET2. The bovine cDNA encodes 177 amino acids of the PPET2 polypeptide, in which a 21-amino acid mature ET2 peptide and a 16-amino acid ET2-like peptide as well as a 23-amino acid putative signal peptide were found. The bovine ET2-like peptide sequence was missing a dibasic amino acid pair at the C-terminal, in contrast to human, mouse and rat, for which the ET2-like sequence is flanked by dibasic pairs at both the N- and C-terminals. Gene expression analysis by RT-PCR showed that the transcript is expressed in various organs including heart, lung, liver, kidney, gastrointestinal tract, uterus and ovary, but not in spleen. Within the gastrointestinal tract, gene expression was detected in rumen, a ruminant-specific digestive organ, as well as stomach, duodenum and colon.
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Uchide et al. (2003) studied this question. Bovine preproendothelin-2 cDNA encodes a 177-amino acid polypeptide expressed in various organs including the heart, lung, liver, kidney, and gastrointestinal tract, but not the spleen.
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