Key result
PAD linked to an ~19-fold increase in visceral adipose IL-6 expression versus healthy controls.
Why the study?
Inflammatory changes in adipose tissue contribute to atherosclerosis development, but gene expression profiles in adipose tissue of patients with peripheral arterial disease were not well characterized.
Observational (n=59)
No
Visceral adipose tissue in patients with peripheral arterial disease exhibits significantly increased pro-inflammatory gene expression compared to healthy controls, suggesting its active role in atherogenesis.
Answers are grounded in this paper and cite their sources.
Visceral adipose inflammation may contribute to PAD; hypothesis-generating and requires prospective validation before clinical consideration.
Inflammatory changes, both in the arterial wall and adipose tissue, play a crucial role in the development of atherosclerosis. We measured the gene expression of tumor necrosis factor-alpha (TNFalpha), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living kidney donors (LKD) and patients with peripheral arterial disease (PAD). Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue (PVAT). Data of PAD patients showed significantly higher expression in VAT in all three genes (TNFalpha 5-fold, p<0.05; MCP-1 3.6-fold, p<0.05; IL-6 18.8-fold, p<0.001). The differences in PVAT and SAT were less significant. Total body pro-inflammatory status was documented by higher TNFalpha concentration in patients (4.86+/-1.4 pg/ml) compared to LKDs (2.14+/-0.9 pg/ml; p<0.001), as was hsCRP (11.8+/-7.0 in PAD; 1.5+/-0.48 in LKDs; p=0.017). We found no age-dependent relationship between gene expression vs. TNFalpha and hsCRP concentrations in both compared groups. No effect of the atherosclerosis score on gene expression and circulating inflammatory markers within the PAD group was observed. Our results suggest that the AT of PAD patients infiltrated with macrophages produces more cytokines involved in the development of inflammation and atherosclerosis.
No takes yet. Share an insight, caveat, or question.
Čejková et al. (2017) conducted an observational in Peripheral arterial disease (atherosclerosis) (n=59). Peripheral arterial disease vs. Healthy living kidney donors was evaluated on IL-6 gene expression in visceral adipose tissue (18.8-fold, p=<0.001). Patients with peripheral arterial disease exhibited significantly higher pro-inflammatory gene expression in visceral adipose tissue, including an 18.8-fold increase in IL-6 compared to healthy donors.
Synapse has enriched 10 related papers. Consider them for comparative context: